Reptiles occupy a special niche within the Niobrara Valley. Turtles are frequently observed while canoeing the Scenic River. Species such as snapping, painted, and spiny softshell turtles can be seen warming themselves on rocks or streambanks on a sunny day.
The ringneck snake occurs in deciduous forest oriented areas of the valley and reaches its western limits there, while the eastern hognose snake also occurs in the valley and is otherwise only marginally distributed across the Sandhills. Others commonly found in the area include prairie rattlesnakes, bull snakes, and red-sided garter snakes.